3D cell culture is an artificially-created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions

3D cell culture is an artificially-created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. Unlike 2D environments (e.g. a petri dish), a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in vivo. These three-dimensional cultures are usually grown in bioreactors, small capsules in which the cells can grow into spheroids, or 3D cell colonies. Approximately 300 spheroids are usually cultured per bioreactor.

Cell-Mate is a biomimetic 3D cell culture matrix designed to support and enhance your 3D cell culture assays. Materials are carefully chosen and processed to create an ideal microenvironment for your cells. Cell-Mate™ is also injectable, offering seamless translation from your incubator to your animal model.

Cell-Mate3D™ is an injectable, tissue-like matrix that offers researchers a biologically relevant microenvironment for in vitro and in vivo biomedical research. It is chemically defined: composed of naturally occurring biopolymers Hyaluronic Acid (HA) and Chitosan (CT).

  • HA is a linear polysaccharide found in the extracellular matrix of connective, epithelial, and neural tissues. It is involved in cell proliferation, migration, embryonic development, and wound healing. HA also plays a role in cancer biology.
  • CT, derived from Chitin, is a positively charged polysaccharide that is found in the exoskeleton of crustaceans.

When Cell-Mate3D™ dry blend is combined with cells in hydration fluid, Cell-Mate3D™ matrix forms instantly through electrostatic interactions between HACOOgroups and CTNH3+ groups. This interaction results in Poly-Electrolytic-Complex fibers that give structure to the construct.

Cell Embedding Process

Advance your research with Cell-Mate3D™

  • Tissue-like stiffness better mimics in vivo extracellular environment
  • Can allow for emergence of complex 3D structures/micro-tissues
  • Cells may secrete own extracellular matrix into construct over time

Advantages of Cell-Mate3D™ over other 3D Culture options

  • Permits long-term 3D cultures without producing a necrotic core
  • No cytotoxic chemical or UV crosslinking required
  • Chemically defined
  • Use both in vitro and in vivo (injectable)
  • Cells distributed throughout the matrix

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